ICE COLD-PCR

Review of ICE COLD-PCR Potential

Jörg Tost (2015): The clinical potential of Enhanced-ice-COLD-PCR, Expert Review of Molecular Diagnostics,
DOI: 10.1586/14737159.2016.1123623

Review of all COLD-PCR Variations

Magdalena Thurin and Francesco M. Marincola (eds.), Molecular Diagnostics for Melanoma: Methods and Protocols, Methods in Molecular Biology, vol. 1102,
DOI 10.1007/978-1-62703-727-3_33

Review of all COLD-PCR Methods

Coren A Milbury; Jin Li; Pingfang Liu; G Mike Makrigiorgos, Expert Rev Mol Diagn. 2011;11(2):159-169.

Protocol for Pyrosequencing analysis of ICE COLD-PCR products

Ulrich Lehmann and Jörg Tost (eds.), Pyrosequencing: Methods and Protocols, Methods in Molecular Biology, vol. 1315, DOI 10.1007/978-1-4939-2715-9_7

Performance Comparison of all COLD-PCR methods using TP53 mutation

Milbury, Coren A., et al. “Ice-COLD-PCR Enables Rapid Amplification and Robust Enrichment for Low-Abundance Unknown DNA Mutations.” Nucleic Acids Research, vol. 39, no. 1, 2011, p. 2.

NRAS in Congenital Melanocytic Nevi

J Invest Dermatol. 2015 Mar;135(3):824-833. doi: 10.1038/jid.2014.437. Epub 2014 Oct 13.

Multiplexing of E-ice-COLD-PCR Assays for Mutation Detection and Identification

Florence Mauger, Antoine Daunay, Jean-François Deleuze, Jörg Tost, Alexandre How-Reagent Clinical Chemistry Aug 2016, 62 (8) 1155-1158; DOI: 10.1373/clinchem.2016.258830

NRAS Mutations with Low Allele Burden have Independent Prognostic Significance for Patients with Lower Risk Myelodysplastic Syndromes

Murphy DM, Bejar R, Stevenson K, et al. NRAS Mutations with Low Allele Burden have Independent Prognostic Significance for Patients with Lower Risk Myelodysplastic Syndromes. Leukemia. 2013;27(10):2077-2081. doi:10.1038/leu.2013.160.

Sensitive Detection of KRAS Mutations Using Enhanced-ice-COLD-PCR Mutation Enrichment and Direct Sequence Identi?cation

Hum Mutat. 2013 Nov;34(11):1568-80. doi: 10.1002/humu.22427. Epub 2013 Sep 17.

Overcoming Acquired Resistance to AZD9291, A Third-Generation EGFR Inhibitor, through Modulation of MEK/ERK-Dependent Bim and Mcl-1 Degradation

Puyu Shi, You-Take Oh, Liang Deng, Guojing Zhang, Guoqing Qian, Shuo Zhang, Hui Ren, Grant Wu, Benjamin Legendre Jr, Emily Anderson, Suresh S. Ramalingam, Taofeek K. Owonikoko, Mingwei Chen and Shi-Yong Sun DOI: 10.1158/1078-0432.CCR-17-1574 Published November 2017

Ultrasensitive detection and identification of BRAF V600 mutations in fresh frozen, FFPE, and plasma samples of melanoma patients by E-ice-COLD-PCR

How-Reagent, A., Lebbé, C., Bousard, A. et al. Anal Bioanal Chem (2014) 406: 5513. https://doi.org/10.1007/s00216-014-7975-5

Comparison of the quantification of KRAS mutations by digital PCR and E-ice-COLD-PCR in circulating-cell-free DNA from metastatic colorectal cancer patients

David Sefrioui, Florence Mauger, Laurence Leclere, Ludivine Beaussire, Frédéric Di Fiore, Jean-François Deleuze, Nasrin Sarafan-Vasseur, Jörg Tost, Clinica Chimica Acta, Volume 465,2017,Pages 1-4,ISSN 0009-8981, https://doi.org/10.1016/j.cca.2016.12.004.

COLD-PCR Enrichment of Rare Cancer Mutations prior to Targeted Amplicon Resequencing

Coren A. Milbury, Mick Correll, John Quackenbush, Renee Rubio, G. Mike Makrigiorgos DOI: 10.1373/clinchem.2011.176198 Published February 2012

Poster 0.01% Level of Mutation Detection with ICE COLD-PCR

Poster Cancer Patient Mutation Analysis in ctDNA & CTCs using ICE COLD-PCR

Poster High Level Mutation Detection using ICE COLD-PCR and NGS

Poster High-Level Mutation Detection using DISSECT & ICE COLD-PCR & NGS Analysis

Poster High-Level Mutational Analysis using BLOCker & ICE COLD-PCR

Poster ICE COLD-PCR ctDNA Analysis using Sanger & NGS

Poster Melanoma Mutation Analysis using ICE COLD-PCR & BLOCker

Poster Monitoring Mutation Changes in Cancer Patients using ICE COLD-PCR

Poster Monitoring Mutation Changes in Melanoma Patients using ICE COLD-PCR

Poster Mutation Analysis of Circulating Tumor Cells using ICE COLD-PCR

Poster Mutation Analysis of Lung Cancer Patients using ICE COLD-PCR

Poster Prostate Cancer Androgen Receptor Analysis by ICE COLD-PCR

Poster Single Thermocycler Plate Multiplexed ICE COLD-PCR

White-Paper Concordance of Clinical Testing using ICE-COLD-PCR